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. 2003 Oct;185(20):5953–5958. doi: 10.1128/JB.185.20.5953-5958.2003

FIG. 1.

FIG. 1.

Immunodetection of PC-PLC and Mpl from broth-grown bacteria. Bacteria were grown in LB-MOPS-G1P or LB-MOPS-glucose (DP-L1075). Bacterial lysates, SDS-βME extracts, and secreted proteins were prepared as described in Materials and Methods. Equivalent amounts of culture OD units were loaded per lane. Proteins were detected by Western immunoblotting. The various polypeptides migrated as indicated on the figure. Lanes 1 to 3 (both panels), 10403S wild-type strain. Negative controls in lane 4 (left panels from the top) include DP-L1935 (ΔplcB), EJ-L12 (ΔinlA), DP-L1552 (ΔplcA), and DP-L1075 (Tn917 insertion in prfA). The negative control in lane 4 (right panel) is DP-L2296 (Δmpl).