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. 2003 Oct;185(20):5953–5958. doi: 10.1128/JB.185.20.5953-5958.2003

FIG. 2.

FIG. 2.

Detection of PC-PLC in the cell wall fraction of broth-grown bacteria. Bacteria were grown in LB-MOPS-G1P or LB-MOPS-glucose (DP-L1075). Bacterial lysates, cell walls, and protoplasts were prepared as described in Materials and Methods. Equivalent amounts of culture OD units were loaded per lane. Proteins were detected by Western immunoblotting. The various polypeptides migrated as indicated on the figure. Lanes: 1 to 3,10403S wild-type strain; 4 to 6, DP-L2296 (Δmpl); 7 (top to bottom), EJ-L12 (ΔinlA), DP-L1935 (ΔplcB), and DP-L1075 (Tn917 insertion in prfA).