FIG. 3.

Behavior of representative nla mutants during development on TPM agar plates. Cells were spotted on TPM starvation agar and monitored visually as described in Materials and Methods. Development of the indicated nla mutants and wild-type strain DK1622 was observed for 5 days using a phase-contrast microscope. The aggregation phenotypes of nla19 and nla23 mutant cells are similar to those of nla1 mutant cells, and the aggregation phenotypes of nla18 and nla28 mutant cells are similar those of nla6 mutant cells. Photographs were taken after 12, 24, 48, 72, and 120 h using a total magnification of ×40.