TABLE 1.

Developmental phenotypes of wild-type and nla mutant strains^a

Strain	Aggre- gationb	Fruiting body spores (% of wild type)c	Glycerol spores (% of wild type)d
DK1622 (wild type)	+	100.0 ± 9.5	100.0 ± 16.2
AG301 (nla1)	+/−	89.4 ± 9.6	83.5 ± 3.8
AG304 (nla4)	−	0.2 ± 0.2	2.4 ± 2.3
AG306 (nla6)	+/−	0.2 ± 0.2	5.1 ± 0.7
AG318 (nla18)	+/−	<0.0002	0.1 ± 0.1
AG319 (nla19)	+/−	107.7 ± 14.6	78.8 ± 5.8
AG323 (nla23)	+/−	129.4 ± 17.6	122.0 ± 9.2
AG324 (nla24)	−	<0.0002	8.9 ± 2.7
AG328 (nla28)	+/−	2.1 ± 1.5	76.8 ± 4.5

^aCells were placed on TPM agar and allowed to develop for 5 days. Development was monitored visually using phase-contrast microscopy. Spore assays were performed three times for each strain. The mean values (± standard deviations) for the spore assays are shown as percentages of DK 1622 (wild type). The number of spores produced by wild-type cells ranged from 3 × 10⁶ to 6 × 10⁶.

^b+, produced normal-looking fruiting bodies; −, failed to produce normal-looking fruiting bodies; +/−, produced normal-looking fruiting bodies but aggregation was delayed.

^cValues were determined by transferring sonication- and heat-resistant spores to CTTYE agar plates, incubating the plates for 5 days, and counting the number of colonies that arose from the spores.

^dValues were determined by counting the number of sonication- and heat-resistant spores by using a Petroff-Hausser chamber and phase-contrast microscopy.