TABLE 2.
Swarm diameters of wild-type and nla strains on 0.4 and 1.5% agara
| Strain | Mean swarm diameter (% of wild type)
|
|
|---|---|---|
| Soft agar (0.4%) | Hard agar (1.5%) | |
| DK 1622 (wild type) | 100 ± 3 | 100 ± 2 |
| DK 2161 (A− S−) | 38 ± 1 | 37 ± 1 |
| AG301 (nla1) | 69 ± 5 | 92 ± 4 |
| AG304 (nla4) | 83 ± 5 | 86 ± 2 |
| AG306 (nla6) | 100 ± 1 | 108 ± 3 |
| AG318 (nla18) | 86 ± 2 | 88 ± 5 |
| AG319 (nla19) | 65 ± 2 | 99 ± 3 |
| AG323 (nla23) | 51 ± 2 | 76 ± 2 |
| AG324 (nla24) | 41 ± 1 | 34 ± 1 |
| AG328 (nla28) | 90 ± 2 | 94 ± 4 |
a
Cells were grown to a density of 5 × 108 cells/ml in CTTYE broth and concentrated 10-fold. A total of 3 μl of concentrated cells was spotted on CTTYE containing 0.4 or 1.5% agar, and the cells were incubated for 5 days at 32°C. The mean diameter (± standard deviation) of five swarms produced by each nla strain was determined and normalized to the mean diameter of five swarms produced by wild-type strain DK1622.