TABLE 3.
Extracellular complementation of asgA, csgA, and nla mutantsa
| Strain mixture
|
Sporulation of test strain (% of wild type) | |
|---|---|---|
| Test strain | Coculture strain | |
| DK1622 (wild type) | 100 ± 12.2 | |
| DK5057 (asgA) | <0.001 | |
| DK5208 (csgA) | <0.001 | |
| AG341 (nla4) | 0.3 ± 0.2 | |
| AG342 (nla6) | 0.2 ± 0.1 | |
| AG343 (nla18) | <0.002 | |
| AG344 (nla28) | 4.0 ± 2.6 | |
| AG341 (nla4) | DK1622 (wild type) | 0.3 ± 0.2 |
| AG342 (nla6) | DK1622 (wild type) | 0.2 ± 0.2 |
| AG343 (nla18) | DK1622 (wild type) | 28.4 ± 6.4 |
| AG344 (nla28) | DK1622 (wild type) | 5.4 ± 3.4 |
| DK5057 (asgA) | DK1622 (wild type) | 88.2 ± 8.9 |
| DK5208 (csgA) | DK1622 (wild type) | 83.8 ± 11.4 |
| DK5057 (asgA) | AG341 (nla4) | 1.2 ± 0.8 |
| DK5208 (csgA) | AG341 (nla4) | 0.2 ± 0.03 |
| DK5057 (asgA) | AG342 (nla6) | 12.7 ± 3.4 |
| DK5208 (csgA) | AG342 (nla6) | 8.3 ± 2.6 |
| DK5057 (asgA) | AG343 (nla18) | 9.6 ± 1.9 |
| DK5208 (csgA) | AG343 (nla18) | 79.3 ± 11.5 |
| DK5057 (asgA) | AG344 (nla28) | 15.9 ± 5.9 |
| DK5208 (csgA) | AG344 (nla28) | 8.3 ± 3.4 |
a
The indicated strains were mixed at a 1:1 ratio and spotted onto TPM agar plates as described in Materials and Methods. The sporulation efficiencies of test strains were determined after 5 days of development as described in Table 1. The mean values for the spore assays were determined from three independent experiments and normalized to the mean value for strain DK1622 alone (wild type for sporulation). The number of spores produced by DK1622 cells ranged from 3 × 106 to 5 × 106. Standard deviations are shown next to the means.