FIG. 2.

SDS-PAGE analysis (10% acrylamide) of KGOR-containing fractions obtained during purification of KGOR. Lanes: S, molecular mass standard; 1, supernatant (100,000 × g centrifugation) cell extract; 2, PEG-precipitated 6% supernatant; 3, PEG-precipitated 11% pellet; 4, DEAE-Sepharose fraction; 5, Q-Sepharose fraction; 6, Reactive Red agarose fraction; 7, gel filtration fraction; 8, gel filtration fraction (1 μg) separated by a SDS-8% PAGE, which was run until the molecular mass standard of ovalbumin nearly reached the front; 9, 67-kDa marker bovine serum albumin. For lanes S and 1 to 7, 8 to 25 μg of protein was applied.