TABLE 2.

General properties of KGOR from A. evansii

Property	Value
Reaction catalyzed	2-Oxoglutarate + CoA + NADP+→succinyl-CoA + CO2 + NADPH + H+
Electron donor	2-Oxoglutarate
Electron acceptors	NADP+ (100%), benzyl viologen (60%), methyl viologen (40%)
Apparent Kms	2-Oxoglutarate, 1.2 mM; CoA, 32 μM; NADP, 16 μM
Sp act	19.3 μmol of 2-oxoglutarate min−1 mg−1
Turnover no.	93 s−1
pH optimum	7.8 (determined in Tris-HCl buffer)
Molecular mass	290 kDa (determination by gel filtration)
Subunits	63 (γ), 62 (α), and 37 (β) kDa (determined by SDS-PAGE)
Suggested composition	(αβγ)2
Iron	24 mol/mol
Acid-labile sulfur	23 mol/mol
Cofactors	FAD, 1.4 mol/mol; TPP, 0.95 mol/mol
Absorption coefficients	ɛ280 = 385,000 M−1 cm−1, ɛ450 = 85,000 M−1 cm−1
Stabilizer	Glycerol (20%)