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(A) Expression and purification of the Fur protein. Lane 1, protein extracts from noninduced E. coli cells harboring plasmid pET15furB; lane 2, protein extracts from cells induced for 3 h with IPTG; lane 3, purified His-tagged Fur protein; lane 4, untagged Fur protein. (B) Western blot analysis showing Fur expression in the wild-type MC58 (lane 1), the MC-Fko fur mutant (lane 2), and the MC-Fko-C complemented mutant (lane 3) strains. Lane M, molecular size standards.
