TABLE 2.
Primers used in this study
| Primer | Sequencea | Siteb |
|---|---|---|
| Eryt-UP | GCAAACTgcAGAGTGTGTTGATAG | PstI |
| Eryt-DO | CCGTAGGCGCTcGaGACCTCTTTAGCTTCTTG | XhoI |
| Ery-DO-C | CAGGTTACTAAAGGGAATGGAG | |
| Fla-UP-L | GGTTCCGTACTAgTTGTACTGTCTGC | SpeI |
| Fla-UP-R | aatttaactcgagCCACCAATCCCACACCACCCTTACC | XhoI |
| Fla-DO-L | ATAAATGTAAAGGaTCCGTTTCATAGCTAAGG | BamHI |
| Fla-DO-R | CGCCGTCAACCCgGgTGCCGAGCTGGAAAAAGAGC | XmaI |
| Fla-UP-C | CTGAAGCAAAGTCGGAAAACGCCGGC | |
| Fla-DO-C2 | CTCGAAACCGGTTCTGACGG | |
| FurP-1 | cggatgaattcTCACGGAAATGCCTTTCTGTGC | EcoRI |
| FurP-2 | attcagggatccCTTCCGCATGCGTCTCGAAC | BamHI |
| Fmb-F | cggatccatATGGAAAAATTCAACAATATTGCAC | NdeI |
| Fmb-R | attcagggatccTTAACGTTTGCCCTTGGCCTG | BamHI |
| Fur-N | attcaggatgcatTCACGGAAATGCCTTTCTGTGC | NsiI |
| FkoB-1 | attcaggaattcGAGCGGTGTCATGTGTGTTCC | EcoRI |
| FkoB-2 | attcagggatccGACGTTATAATACGCAATTTCGGCC | BamHI |
| FkoB-3 | attcagggatccCCGGACGGTTTGTTGTTCAGAC | BamHI |
| FkoB-4 | attcagctgcagGAATGCGCGTACCCCATTTCG | PstI |
| FkoB-5 | CGGGATGGTTGTTGACGGC | |
| FkoB-6 | CGTTTCACCGCTTTCATCGGG | |
| Lac-DO-C | CGCTACCATTACCAGTTGGTCTGG | |
| LacZ-PE | TAGCAGGCTCTTTCGATCC | |
| Upf-N2 | attcagatgcatGGTAACCTTCAGACCGCTGTC | NsiI |
| Upf-S | attcaggcatgcTCACGGAAATGCCTTTCTGTGC | SphI |
| Us1 | CCAGCGGTCGGTATGGAATGCG | |
| Us2 | CGATGCGCGGACAGGGCggatccCGCTTAATG | BamHI |
a
Capital letters indicate N. meningitidis-derived sequences, italicized capital letters indicate E. coli-derived sequences, lowercase letters indicate sequences added for cloning purposes, and underlined letters indicate recognition sites.
b
Restriction enzyme sites added for cloning purposes.