FIG. 2.

DNase I footprinting of wild-type DctD and mutant forms of DctD_Δ1-142 in the presence of ATP or ATP analogs. DctD_{Δ1-142,T223I} (lanes 2 to 6), wild-type DctD (lanes 7 to 11), and DctD_{Δ1-142,R305Q} (lanes 12 to 16) were examined in a DNase I footprinting assay. Reaction mixtures contained 800 nM concentrations of monomers of the indicated DctD proteins. Lane 1, no-protein control; lanes 2, 7, and 12, no addition of nucleotide; lanes 3, 8, and 13, addition of 3 mM ATP; lanes 4, 9, and 14, addition of 3 mM ATPγS; lanes 5, 10, and 15, addition of 2 mM ADP; and lanes 6, 11, and 16, addition of 2 mM ADP, 4 mM NaF, and 0.1 mM AlCl₃. The bands marked with arrows correspond to bands 1, 2, and 3 in Fig. 1.