TABLE 1.
Quantitation of DNase I footprinting assays with DctDΔ1-142
| Nucleotide | Relative intensitya
|
||||
|---|---|---|---|---|---|
| Band 1 | Band 2 | Band 3 | Band A | Band B | |
| None | 1 | 1 | 1 | 1 | 1 |
| ATP | 0.31 | 3.03 | 3.45 | 0.87 | 0.75 |
| ATPγS | 0.85 | 0.79 | 0.96 | 0.73 | 0.84 |
| ADP | 0.81 | 0.79 | 1.05 | 0.78 | 0.83 |
| ADP-AlFx | 0.25 | 2.63 | 2.42 | 0.78 | 0.74 |
a
Bands correspond to those indicated in Fig. 1. For each band, the intensity is relative to that of the corresponding band in the no-nucleotide control. Values that are greater than 1 indicate enhanced cleavage relative to the control, while values that are less than 1 indicate increased protection relative to the control.