FIG. 2.

Verification of the internal deletion in otsA and otsB. Southern blots of genomic DNA from T. thermophilus RQ-1 and two kanamycin-resistant clones (RQ-1M6 and RQ-1M8) are shown. Bands were identified by hybridization with a 2.05-kbp fragment corresponding to otsA and otsB (A) or kat (B). Lanes 1, 4, and 7, RQ-1M6; lanes 2, 5, and 8, RQ-1M8; lanes 3, 6, and 9, RQ-1. (C) Genetic organization of trehalose-synthesizing genes in the chromosomes of the wild type and an RQ-1 deletion mutant.