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. 1998 Apr 14;95(8):4415–4418. doi: 10.1073/pnas.95.8.4415

Figure 2.

Figure 2

Identification of the pair of homologous chromosomes on which ZOV3, IREBP, and EE0.6 sequences are colocalized and partial morphological differentiation of the W chromosome in the ostrich. Chromosome pairs 1–6 from in situ-hybridized metaphase sets of the female emu (A) and the male ostrich (B) are shown. Signals of FISH are colocalized on chromosome 5 in the emu and chromosome 4 in the ostrich. Fluorescence signals were produced with the following probes: biotinylated pEGIREBP (arrowhead) and DIG-pEGEE0.6 (arrow) in A and DIG-pOGIREBP (arrowhead) and biotinylated pOGZOV3 (triangle) in B. (C) Pairs of chromosome 4 of the ostrich were taken from in situ-hybridized metaphase sets of the male, the female, and the individuals (nos. 7 and 8) of unknown gender. Fluorescence signals shown were produced with biotinylated pOGZOV3 (triangle) and DIG-labeled pOGIREBP (arrowhead). Partial differentiation of the W chromosome in the female and the no. 8 ostriches is noted by shortening of the arm from which the IREBP gene is missing.