Figure 3.
Southern blot hybridization suggesting the absence of the IREBP gene on one of the homologous chromosomes in the female ostrich. The BglII-digested genomic DNA samples (5 μg/lane) from the male and female ostriches and from the two ostriches (nos. 7 and 8) of unknown gender as indicated were subjected to hybridization with the following 32P-labeled probes: (A) a 2.4-kb XbaI fragment of pOGEE0.6; (B) a 2.7-kb EcoRI–BglII fragment of pOGIREBP; and (C) a 3.5-kb BamHI fragment of pOGZOV3. The same blot was hybridized and rehybridized in this order. Relative intensities of hybridization signals are indicated.