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Sequences of core promoters used in this study. −10 and −35 hexamer sequences are in bold. The +1 NTP is in bold capital letters. Sequence endpoints of DNA fragments used to construct promoter-lacZ fusions are indicated in Table 1. The rrnB P1(dis) promoter (24) is identical to rrnB P1 except for a 3-bp substitution (underlined) that causes the promoter to form a longer-lived open complex, resulting in a loss of regulation (3). This promoter makes the same transcript as rrnB P1.
