FIG. 1.
Nucleotide sequences of the FF(−n)FF(−41.5) and FF(−n)FF(−61.5) promoter series. (a) The FF(−n)FF(−41.5) promoter series. The upper part of the figure shows the complete sequence of the FF(−41.5) promoter from the EcoRI site (GAATTC) to the HindIII site (AAGCTT) located at position +36 relative to the transcription start point (+1, in boldface). The FNR consensus binding site (FF; TTGAT-n4-ATCAA) is shaded. The −10 sequence (CATAAT) is underlined, and the BamHI site (GGATCC) located immediately downstream of the FNR binding site is underlined twice. The lower part of the panel shows the sequences of the FF(−n)FF(−41.5) promoter series, aligned at the FF site centered around position −41.5. The name of each promoter reflects the location of the upstream FF site. The full sequences between the EcoRI site (GAATTC) and position −27, showing the BglII/BamHI hybrid site (AGATCC), are shown. The promoter sequences are identical to the FF(−41.5) sequence between position −27 and the HindIII site. (b) The FF(−n)FF(−61.5) promoter series. The upper part of the panel shows the complete sequence of the FF(−61.5) promoter from the EcoRI site to the HindIII site located at position +36 relative to the transcription start point (+1, in boldface). The FNR consensus binding site is shaded, the −10 sequence is underlined, and the BamHI site is underlined twice. The lower part of the panel shows the sequences of the FF(−n)FF(−61.5) promoter series, aligned at the FF site centered around position −61.5. The name of each promoter reflects the location of the upstream FF site. The full sequences between the EcoRI site and position −27, showing the BglII/BamHI hybrid site, are shown. The promoter sequences are identical to the FF(−61.5) sequence between position −27 and the HindIII site.