FIG. 3.
Analysis of promoter binding by FNR D154A. For DNaseI footprint analysis, promoter fragments were labeled with [γ-32P]ATP, were incubated with various concentrations of FNR D154A prior to treatment with DNaseI, and were analyzed on a DNA sequencing gel. The gel was calibrated with Maxam-Gilbert G+A sequencing reactions. Regions of protection due to FNR D154A are indicated with gray rectangles. The samples were loaded as follows: G+A sequencing reaction (M); no DNaseI, 3 μM FNR D154A control (lanes 1, 5, and 9); DNaseI, no protein control (lanes 2, 6, and 10); DNaseI, 0.1 μM FNR D154A (lanes 3, 7, and 11); DNaseI, 3 μM FNR D154A (lanes 4, 8, and 12).