TABLE 4.
FNR-dependent transcription using FF(−n)FF(−41.5) and EE(−n)FF(−41.5) promoters
| Promotera | Relative activity (%)b of:
|
|||
|---|---|---|---|---|
|
E. coli M182 (fnr+)
|
E. coli M182 fnrA1 (lacking fnr)
|
|||
| Activity | Error | Activity | Error | |
| FF(−85.5)FF(−41.5) | 11 | 1 | 8 | 2 |
| EE(−85.5)FF(−41.5) | 97 | 20 | 7 | 2 |
| FF(−90.5)FF(−41.5) | 139 | 11 | 7 | 0.4 |
| EE(−90.5)FF(−41.5) | 102 | 15 | 6 | 2 |
a
Promoters were cloned on EcoRI-HindIII fragments in the lac expression vector pRW50.
b
Cells were grown anaerobically in LB supplemented with 35 μg of tetracycline ml−1 and 0.2% fructose, and β-galactosidase activity was determined as described in Materials and Methods. Activity is shown relative to the β-galactosidase activity obtained using the FF(−41.5) promoter in the M182 background and is the means of three independent determinations. The error is one standard deviation from the mean.