Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Localization of Puc2BA-PhoA fusion proteins in R. sphaeroides mutant ΔPUC2BA containing plasmids pUC2P-PhoA/pRK415 (2P-PhoA), pUC2B-PhoA/pRK415 (2B-PhoA), pUC2AS-PhoA/pRK415 (2AS-PhoA), and pUC2A-PhoA/pRK415 (2A-PhoA). Cells were grown anaerobically at 100 W/m2 to an OD600 of 0.3 to 0.4. Fifty micrograms of protein was applied to each lane of an SDS-PAGE gel. Membranes were isolated as described in Materials and Methods. The sizes of the hybrid proteins are indicated on the right.
