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. 2003 Oct;185(20):6171–6184. doi: 10.1128/JB.185.20.6171-6184.2003

FIG. 4.

FIG. 4.

Immunoprecipitation of Puc2BA-PhoA of R. sphaeroides mutant strain ΔPUC2BA containing plasmids pUC2B-PhoA (2B-PhoA), pUC2AS-PhoA (2AS-PhoA), and pUC2A-PhoA/pRK415 (2A-PhoA). Mutant strain ΔPUC2BA containing plasmid pRK415 was used as a control. (A) Tricine-SDS-PAGE of immunoprecipitated complexes. The stained bands corresponding to the chimeric proteins Puc2A-PhoA, Puc2AS-PhoA, and Puc2A-PhoA are indicated by arrows. (B) Absorption spectra of the immunoprecipitated complexes and purified LH2 complex from mutant strain ΔPUC2BA (solid line). The arrows indicate the absorption peaks for B800 and B850 of LH2, respectively.