TABLE 2.
Primers used in this study for operon fusion construction
Plasmid | Primers | Cloned sequence(s) |
---|---|---|
pUC2P-2B1A | P1 (5′-CCAAGCTTGGATCGTGCTCTGCCGGCTC-3′) and P2 (5′-CATCTTTACTTCTCCCTCAG-3′) | puc2P and puc2B |
P3 (5′-CTGAGGGAGAAGTAAAGATGACCAACGGCAAAATCT-3′) and P4 (5′-CCGGTACCGAGTAGCCTTTTGCACCTCC-3′) | puc1A | |
pUC1P-2B1A | P5 (5′-CCAAGCTTCCCGCGTGCTGATCTCGGCC-3′) and P6 (5′-CACTGTGTCGTCTCCCAACT-3′) | puc1P |
P4 and P7 (5′-AGTTGGGAGACGACACAGTGACCGATGATCCGAAAAA-3′) | puc2B and puc1A | |
pUC2P-1BA | P1 and P8 (5′-TTGTACTCTCCCGAATGTGC-3′) | puc2P |
P4 and P9 (5′-ACATTCGGGAGAGTACAAGTGACTGACGATCTGAAC-3′) | puc1BA | |
pUC1P-1B2AS | P5 and P10 (5′-CATGTCAGTCTTCTCCTATC-3′) | puc1P and puc1B |
P11 (5′-GATAGGAGAAGACTGACATGAACAACTCGAAGATGTG-3′) and P12, (5′-CCGGTACCGGCCACGGCTACGAGCCCTG-3′) | First 144 bp of puc2A DNA (puc1A-like) | |
pUC1P-1B2A | P5 and P10 | puc1P and puc1B |
P11 and P13 (5′-CCGGTACCGGTGGAACACCGGCCACAAC-3′) | puc2A |