TABLE 3.
Light-harvesting complex LH2 in R. sphaeroides 2.4.1 and mutants of this straina
| Strain | Amt of LH2 complex (nmol/mg of protein)
|
||
|---|---|---|---|
| 100 W/m2 | 10 W/m2 | 3 W/m2 | |
| 2.4.1 | 13.0 | 26.0 | 36.3 |
| ΔPUC2B | 9.5 | 18.3 | 29.0 |
| ΔPUC2AS | 12.0 | 23.1 | 33.9 |
| ΔPUC2A | 11.9 | 24.0 | 34.1 |
| ΔPUC2BAS | 8.8 | 15.9 | 27.0 |
| ΔPUC2BA | 8.8 | 16.6 | 26.2 |
| ΔPUC1B | 0.8 | 1.1 | 0.9 |
| ΔPUC1A | NDb | ND | 0.6 |
| ΔPUC1BA | ND | 0.1 | 0.3 |
| ΔPUC-C | ND | ND | 0.3 |
| ΔPUC1BA-C | ND | 0.4 | 0.5 |
| ΔPUC1BA-2BA | 0.2 | 0.2 | ND |
| ΔPUC1BA-2BA-C | ND | ND | 0.1 |
Cells were grown anaerobically in the light to an OD600 of 0.3 to 0.4. Three independent experiments were performed, and the standard deviations were within ±10%. The values are averages for the three independent experiments.
ND, not detectable.