TABLE 4.
β-Galactosidase activities of cell extracts of R. sphaeroides 2.4.1 and mutant derivatives of this strain containing the pCF200km (puc1BAC::lacZ) transcriptional fusion plasmida
| Strain | β-Galactosidase activity (U/min/mg of protein)
|
||
|---|---|---|---|
| 100 W/m2 | 10 W/m2 | 3 W/m2 | |
| 2.4.1 | 1,038 | 2,043 | 3,121 |
| ΔPUC2BA | 1,121 | 2,197 | 3,213 |
| ΔPUC1BA | 1,029 | 2,098 | 3,191 |
| ΔPUC-C | 381 | 593 | 1,127 |
| ΔPUC1BA-C | 410 | 506 | 1,092 |
| ΔPUC1BA-2BA-C | 379 | 510 | 1,009 |
| ΔPUC-C (pUC-1C) | 827 | 1,903 | 2,527 |
| ΔPUC1BA-C(pUC-1C) | 902 | 1,706 | 2,592 |
| ΔPUC1BA-2BA-C(pUC-1C) | 879 | 1,810 | 2,409 |
a
Cells were grown anaerobically in the light to an OD600 of 0.3 to 0.4. Three independent experiments were performed, and the standard deviations were within ±10%. The values are averages for the three independent experiments rounded off to the nearest integer.