TABLE 5.
β-Galactosidase activities of cell extracts of R. sphaeroides 2.4.1 and mutant derivatives of this strain containing the puc1B::lacZ (pUI-1B523A), puc1A::lacZ (pUI1A523A), or puc1C::lacZ (pUI1C523A) translational fusion plasmida
| Strain | Plasmid | β-Galactosidase activity (U/min/mg of protein)
|
||
|---|---|---|---|---|
| 100 W/m2 | 10 W/m2 | 3 W/m2 | ||
| 2.4.1 | pUI1B523A | 640 | 978 | 1,234 |
| pUI1A523A | 546 | 712 | 866 | |
| pUI1C523A | 48 | 91 | 221 | |
| ΔPUC2BA | pUI1B523A | 601 | 918 | 1,381 |
| pUI1A523A | 519 | 691 | 871 | |
| pUI1C523A | 51 | 93 | 226 | |
| ΔPUC1BA | pUI1B523A | 550 | 992 | 1,377 |
| pUI1A523A | 532 | 627 | 829 | |
| pUI1C523A | 51 | 85 | 233 | |
| ΔPUC-C | pUI1B523A | 84 | 222 | 351 |
| pUI1A523A | 104 | 173 | 247 | |
| pUI1C523A | 45 | 73 | 174 | |
a
Cells were grown anaerobically in the light to an OD600 of 0.3 to 0.4. Three independent experiments were performed, and the standard deviations were within ±10%. The values are averages for the three independent experiments rounded off to the nearest integer.