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Genetic and restriction endonuclease cleavage maps of the mobilization regions of plasmids pTC-F14 and pTF-FC2. (A) The 5.55-kb BamHI-XbaI region of pTC-F14 showing the locations of the mob, repB, and pas genes as well as the oriT gene. The positions of the primers used to amplify and construct certain subclones are shown by short horizontal arrows. (B) The previously reported mob region of pTF-FC2 and subclones (20) used in this study.
