TABLE 2.
Mobilization frequency of plasmids and constructs
| Test plasmida | Plasmid present in transa | Mobilization frequency of test plasmidb |
|---|---|---|
| pTC-F14Km | (2.7 ± 1.5) × 10−3 | |
| pTC-F14Cm | (2.8 ± 1.8) × 10−3 | |
| pTC-F14Cmc | R751 | 1.3 × 10−5 |
| pTC-F14Cmc | R388 | <10−6 |
| pTF-FC2 (pDER412) | ≥10 | |
| pTF-FC2 (pDER412) | pTC-F14Km | ≥10 |
| pTC-F14Km | pTF-FC2 (pDER412) | 8.4 ± 0.52 |
| pMmob (F14, mobEDCBA repB pasA) | ≥10 | |
| pMmob1 (F14, mobEDCBA repB) | ≥10 | |
| pMmob5 (F14, mobEDCBA) | (3.3 ± 3.1) × 10−1 | |
| pMmob6 (F14, mobDCBA) | (1.2 ± 1.0) × 10−1 | |
| pMmob7 (F14, mobCBA) | (5.4 ± 3.3) × 10−3 | |
| pMmob8 (F14, mobBA) | <10−6 | |
| pMmob9 (F14, mobEDCB) | <10−6 | |
| pMmob8 (F14, mobBA) | pTC-F14Cm | 2.4 ± 1.4 |
| pMmob1184 (as for pMmob1) | (2.1 ± 0.8) × 10−1 | |
| pMmob1322 (as for pMmob1) | (2.3 ± 1.6) × 10−1 | |
| pTC-F14Cm | pAC105 (FC2, mobEDC) | (7.9 ± 2.1) × 10−1 |
| pTC-F14Cm | pAC209 (FC2, mobDCBA) | (2.3 ± 3.5) × 10−3 |
| pTC-F14Cm | pAC221 (FC2, mobBA) | (8.9 ± 2.4) × 10−4 |
| pTC-F14Cm | pmobE (FC2) | (4.4 ± 2.1) × 10−3 |
| pTC-F14Cm | pmobDE (FC2) | ≥10 |
| pTC-F14Cm | pmobCDE (FC2) | (4.7 ± 2.9) × 10−1 |
| pTC-F14Cm | pAC218 (FC2 mobC) + pmobE (FC2) | (1.1 ± 2.6) × 10−3 |
| pOriTF14 | <10−6 | |
| pOriTF14 | pTC-F14Cm | 1.5 ± 1.0 |
| pOriTF14 | pTF-FC2 (pDER412) | (3.5 ± 0.1) × 10−2 |
| pOriTF14 | pTC-F14Km + pTF-FC2 (pDER412) | ≥10 |
| pOriTFC2 | <10−6 | |
| pOriTFC2 | pTC-F14Cm | <10−6 |
| pOriTFC2 | pTF-FC2 (pDER412) | ≥10 |
| pOriTFC2 | pTC-F14Km + pAC105 (FC2, mobEDC) | (3.5 ± 4.7) × 10−1 |
| pOriTFC2 | pTC-F14Km + pAC221 (FC2, mobBA) | (1.7 ± 0.8) × 10−2 |
a
Where relevant, genes or plasmids are indicated in parentheses.
b
Mobilization frequency is the number of transconjugants per donor during a 60-min mating with a donor/recipient ratio of 1:10 using E. coli S17-1 as the donor and E. coli CSH56 as the recipient. A mating frequency of ≥10 is indicated when the number of transconjugants equaled the number of recipients. Mating frequencies were the average of at least three independent experiments, and standard deviations are indicated.
c
E. coli HB101 was used as the donor strain.