Table 2.
Transfection of the NeoR retrofitted HPRT containing BAC/YACs into A9 mouse cells
| Size of BAC/YAC | Method of transfection | Frequency of NeoR transfections × 10−6 per μg BAC/YAC DNA | Number of NeoR transfectants expressing HPRT* |
|---|---|---|---|
| 80 kb | Lipofection | 38.0 | 10/10 |
| Electroporation | 0.2 | 0/2 | |
| Calcium phosphate | 0.4 | ND | |
| 100 kb | Lipofection | 36.0 | 10/10 |
| Electroporation | 0.01 | ND | |
| 120 kb | Lipofection | 50.0 | 10/10 |
| Electroporation | 1.0 | 5/5 | |
| Calcium phosphate | 16.0 | 5/5 | |
| 140 kb | Lipofection | 75.0 | 10/10 |
| Electroporation | 0.8 | 5/5 | |
| 110 kb (BRCA2)† | Lipofection | 35.0 | 0/10 |
ND, not determined.
*Capable to grow on hypoxanthine/aminopterin/thymidine medium.
†
Human BRCA2 YAC (1) was retrofitted into BAC/NeoR and used as a control in transfection experiments.