Table 1.
Free running circadian locomotor activity periods of various per transformants at different temperatures based on spectral analysis (24)
| Line | 18°C
|
25°C
|
29°C
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| N | n | Period, h | N | n | Period, h | N | n | Period, h | |
| mel | |||||||||
| 2a | 61 | 41 | 24.6 ± 0.1 | 25 | 23 | 25.0 ± 0.1 | 53 | 45 | 24.8 ± 0.1 |
| 17a | 59 | 31 | 24.1 ± 0.2 | 24 | 20 | 24.3 ± 0.7 | 46 | 26 | 24.2 ± 0.2 |
| 34a | 51 | 30 | 24.2 ± 0.2 | 47 | 39 | 25.0 ± 0.1 | 32 | 32 | 24.9 ± 0.2 |
| 116a | 50 | 41 | 23.5 ± 0.1 | 33 | 30 | 25.5 ± 0.1 | 33 | 31 | 25.4 ± 0.2 |
| Pooled | 221 | 143 | 24.1 ± 0.1 | 129 | 112 | 25.0 ± 0.1 | 164 | 134 | 24.9 ± 0.1 |
| mps1 | |||||||||
| I20 | 26 | 17 | 29.4 ± 0.8 | 31 | 18 | 28.3 ± 1.2 | 61 | 13 | 29.0 ± 1.2 |
| I26 | 25 | 21 | 27.4 ± 0.6 | 37 | 17 | 28.9 ± 0.8 | 82 | 8 | 29.5 ± 1.0 |
| Pooled | 51 | 38 | 28.3 ± 0.5 | 68 | 35 | 28.6 ± 0.6 | 143 | 21 | 29.2 ± 0.8 |
| mps2 | |||||||||
| 6 | 31 | 4 | 22.7 ± 4.1 | 61 | 5 | 33.0 ± 1.6 | 65 | 5 | 29.8 ± 1.6 |
| 9 | 28 | 6 | 24.6 ± 2.1 | 29 | 8 | 28.6 ± 2.0 | 39 | 7 | 28.6 ± 2.7 |
| 22 | 28 | 11 | 27.5 ± 2.2 | 68 | 8 | 28.1 ± 2.7 | 34 | 5 | 28.8 ± 2.4 |
| Pooled | 87 | 21 | 25.8 ± 1.5 | 158 | 21 | 29.5 ± 1.3 | 138 | 17 | 29.0 ± 1.3 |
| mps3 | |||||||||
| 65c | 19 | 15 | 24.1 ± 0.1 | 44 | 42 | 25.7 ± 0.1 | 15 | 13 | 25.6 ± 0.2 |
| 67a | 22 | 15 | 23.3 ± 0.2 | 32 | 32 | 24.4 ± 0.1 | 12 | 12 | 25.0 ± 0.2 |
| Pooled | 41 | 30 | 23.7 ± 0.1 | 76 | 74 | 25.1 ± 0.1 | 27 | 25 | 25.3 ± 0.2 |
| mps4 | |||||||||
| 6f | 17 | 15 | 24.3 ± 0.1 | 23 | 30 | 24.8 ± 0.1 | 66 | 62 | 24.8 ± 0.1 |
| 16b | 27 | 16 | 23.9 ± 0.1 | 20 | 17 | 25.1 ± 0.2 | 24 | 21 | 24.7 ± 0.1 |
| Pooled | 44 | 31 | 24.1 ± 0.1 | 43 | 37 | 25.0 ± 0.1 | 90 | 83 | 24.8 ± 0.1 |
| mps5 | |||||||||
| 23 | 25 | 20 | 24.0 ± 0.1 | 68 | 65 | 27.2 ± 0.1 | 51 | 48 | 28.3 ± 0.2 |
| 25 | 67 | 47 | 24.9 ± 0.3 | 73 | 48 | 30.9 ± 0.7 | 97 | 74 | 38.9 ± 1.0 |
| Pooled | 92 | 67 | 24.6 ± 0.2 | 141 | 113 | 28.8 ± 0.4 | 148 | 122 | 34.7 ± 0.8 |
| per01 | 31 | 5 | 22.6 ± 1.1 | 32 | 3 | 19.3 ± 1.9 | 57 | 4 | 21.5 ± 2.1 |
Results based on autocorrelation-derived periods are almost identical (data not shown, but see Fig. 2). Two-way ANOVA omitting the largely arrhythmic mps2 transformants revealed significant effects of temperature (T), genotype (G), and T × G interaction (P < 0.001 in all cases). The Newman–Keuls a posteriori procedure revealed significant changes in period over temperature for both lines of the mps5 transformants (P < 0.002 for both lines). N, number of males examined; n, number of significant rhythmic males. Results are mean ± SEM.