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Immunohistology of frozen thymic sections from C57BL/6 (A), C2D (B), wild-type reconstituted tg (C), and EA137/VA142 tg (D) mice. Sections are stained for I-Ab (immunoperoxidase staining in brown); counterstaining with hematoxylin reveals thymocytes (staining in blue). FACS profiles of gated B220+ splenocytes stained for I-Ab (FITC): E, C57BL/6; F, wild-type reconstituted tg; G, EA137/VA142 mutant tg. The MFIs (X̄ = mean) for B220+ I-A+ lymphocytes are given.
