Figure 2.

RT-PCR analysis of HLA-G mRNA isoforms in an ex vivo skin melanoma metastasis biopsy. The pan-HLA-G primers 257 and 3GU were used for RT-PCR amplification of HLA-G transcripts from an ex vivo skin metastasis (MEL) and from healthy skin biopsy (HS) obtained from the same patient. JEG-3 and first trimester trophoblast (TRO) were used as controls of high HLA-G transcription. HLA-G-specific bands were revealed by hybridization with the GR-specific probe located in exon 2. Bands corresponding to HLA-G1, G2, G3, G4, and G5 transcripts are indicated by arrows. PCR products co-amplified in the same reaction by β-actin primers were detected on the same membrane by a β-actin probe.