Table 3.

Responses of CD8⁺ T hybridomas generated by vaccination with the MBP-specific CD4⁺Vβ8⁺ syngeneic T cell clone 1AE10

					Response
Stimulators					T cell hybridoma 21-5A9					T cell hybridoma 176-5F7
Name	Specificity	Vβ	H-2	Qa-1	Exp. 1	Exp. 2	Exp. 3	Exp. 4	Exp. 5	Exp. 1	Exp. 2	Exp. 3	Exp. 4	Exp. 5
1AE10	MBP	8.3	u	a	2+	0.461	0.846	0.324	0.309	2+	0.415	0.513	0.178	0.248
1AC3	MBP	8.2	u	a	2+	0.377	0.580	0.120	0.264	±	0.060	0.054	0.000	0.037
3AC3	MBP	8.3	u	a	2+	NT	0.242	0.040	0.293	2+	NT	0.209	0.043	0.223
SEB-Vβ8	SEB	8.2	u	a	1+	0.508	0.731	0.416	0.208	2+	0.514	0.637	0.386	0.222
3AB3	MBP	6	u	a	±	0.164	0.127	0.055	NT	0	0.062	0.041	0.027	NT
3AF9	MBP	6	u	a	0	0.080	0.071	0.039	NT	0	0.183	0.117	0.068	NT
3AG2	MBP	6	u	a	0	0.131	0.054	0.043	NT	2+	0.478	0.534	0.314	NT
3BB8	MBP	6	u	a	0	0.172	0.101	0.082	NT	0	0.212	0.178	0.098	NT
LBRM	—	8.2	k	a	2+	0.844	1.434	0.926	0.611	2+	0.923	1.285	0.846	0.611
J1-9f	—	8.2	HLA	b	0	NT	0.114	0.096	0.028	0	NT	0.019	0.018	0.007
4G4-6B5	—	8.2	k	b	0	NT	0.106	0.079	0.017	0	NT	0.086	0.082	0.017
PI	—	—	—	—	3+	1.019	1.497	0.972	0.739	3+	0.782	1.268	0.484	0.327

The results for experiment 1 represent the visual estimates of the responses derived by counting the numbers of blue cells in each well containing 5 × 10⁴ cells after fixation and incubation of the hybridomas with X-Gal (0 = <20; ± = <100; 1+ = 100–1,000; 2+ >1,000; and 3+ = virtually all). The results for experiments 2–6 represent the means minus the background (CD8 cells alone) of a colorimetric assay of β-galactosidase activity. NT, not tested. PI indicates the culture of the hybridomas for 4 hr with 20 ng/ml phorbol 12,13-dibutyrate and 0.4 μM ionomycin.