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Flow cytometric analysis of CBTL after PKH-26 staining. CBTL were purified and stained with PKH-26 on day 0 of primary MLC. They are identified as a homogeneously PKH-26bright population (A). After allostimulation and culture for 7–10 days, a major population of PKH-26dim CBTL is detectable, together with a residual minor population of PKH-26bright cells (B). PKH-26 staining was performed in all MLC experiments.
