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In vitro PI-3K assay after secondary stimulation of alloantigen-primed PKH-26dim CBTL and PKH-26dim PBTL. Cells were stimulated by CD28 cross-linking or with alloantigen and lysed, and immunoprecipitation with anti-CD28 antibodies was performed. The PI-3K activity of the immunoprecipitate was then measured as the amount of radioactive PIP3 generated. Control immunoprecipitation was performed with anti-CD3 antibodies.
