Figure 1.

Venous plasma was pooled from 3–4 male mice of the indicated genotype, and lipoproteins were isolated by ultracentrifugation. The wild-type mice were age- and sex-matched C57BL/6 mice. The top fraction (density < 1.215 g/liter) was subjected to FPLC using a Superose 6 column (Sigma). Thirty-five fractions were collected, and plasma cholesterol and triglycerides were measured in each fraction as described in Materials and Methods.