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. 1998 Apr 14;95(8):4550–4555. doi: 10.1073/pnas.95.8.4550

Table 2.

Cell yields at 14 days in three serial assays with MTX treatment in the 3° assay

Assay No. SA′ cells seeded MTX, nm (3–6 day) 2% CS in 1°
10% CS in 1°
SA′ std. pass. A′ std. pass.
No. of cells (×10−5)
105 0 6.28 20.1*
105 0 55.1 35.3
105 0 50.2 38.0 5.72 5.93
105 200 6.16 3.80 4.00
103 (105) 0 20.1 12.1
103 (105) 200 3.92 3.75

The procedure was the same as that of Table 1 but counts were made of the number of cells at the end of each assay. In addition, during the 3° assay, parallel 1° assays with cell counts were made of the standard passage of the SA′ and A′ cells. 

*

The high value for cell numbers in the 10% CS 1° assay results from the high serum concentration rather than transformation. Because 2% CS is used in all subsequent assays, high cell numbers reflect the extent of cell transformation. 

The assays of the standard passage SA′ were 1° assays but they were made in parallel with the 3° assay of SA′ cells. 

103 (105): in the 3° serial assay of SA′ cells, 103 were mixed with 105 A′ cells, which served as a background for display of focus formation by the 3° SA′ cells.