Table 2.
Assay | No. SA′ cells seeded | MTX, nm (3–6 day) | 2% CS in 1°
|
10% CS in 1°
|
SA′ std. pass. | A′ std. pass. |
---|---|---|---|---|---|---|
No. of cells (×10−5) | ||||||
1° | 105 | 0 | 6.28 | 20.1* | ||
2° | 105 | 0 | 55.1 | 35.3 | ||
3° | 105 | 0 | 50.2 | 38.0 | 5.72† | 5.93† |
105 | 200 | 6.16 | 3.80 | 4.00† | — | |
103 (105)‡ | 0 | 20.1 | 12.1 | |||
103 (105)‡ | 200 | 3.92 | 3.75 |
The procedure was the same as that of Table 1 but counts were made of the number of cells at the end of each assay. In addition, during the 3° assay, parallel 1° assays with cell counts were made of the standard passage of the SA′ and A′ cells.
The high value for cell numbers in the 10% CS 1° assay results from the high serum concentration rather than transformation. Because 2% CS is used in all subsequent assays, high cell numbers reflect the extent of cell transformation.
The assays of the standard passage SA′ were 1° assays but they were made in parallel with the 3° assay of SA′ cells.
103 (105): in the 3° serial assay of SA′ cells, 103 were mixed with 105 A′ cells, which served as a background for display of focus formation by the 3° SA′ cells.