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. 1998 Apr 14;95(8):4567–4572. doi: 10.1073/pnas.95.8.4567

Figure 4.

Figure 4

In vitro kinase assay of FGFR2 receptors. Constructs encoding FGFR2 wild-type or mutant receptors were transiently transfected into COS-1 cells. The cells were lysed, material was immunoprecipitated with FGFR2 antiserum, and an in vitro autophosphorylation reaction was performed in the presence of radiolabeled ATP. (A) Radiolabeled proteins were resolved on a SDS/PAGE 4–12% gradient gel and detected by autoradiography. (B) Identical aliquots were electrophoresed on a SDS/PAGE 4–12% gradient gel, after which proteins were transferred to nitrocellulose, subjected to immunoblotting using the anti-phosphotyrosine antiserum 4G10, and visualized via enhanced chemiluminescence. Monomeric and dimeric species of FGFR2 are indicated. Molecular mass markers in kDa are also indicated. Lanes: 1, mock-transfected cells; 2, wild type; 3, C278F; 4, C342Y; 5, (C278A, C342A); 6, W290G; 7, (W290G, C278A, C342A); 8, T341P; 9, (T341P, C278A, C342A).