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Nondenaturing PAGE of wild-type and mutant Hsp21 showing oxidation-dependent structural changes of the Hsp21 oligomer. The mutants with methionines substituted by leucine, Hsp21-M(49,52,55,59)L and Hsp21-M(49,52,55,59,62,67)L, are referred to as -4M, and -6M. The mutants with cysteine replaced by alanine, Hsp21-C151A and Hsp21-M(49, 52,55,59,62,67)L,C151A, are referred to as -C and -6M-C.
