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Direct titration of [5F]Hir54–65(SO3−) with human prothrombin. The change in fluorescence polarization of a solution containing 100 nM [5F]Hir54–65(SO3−) titrated with prothrombin (Pro) was performed in TBS at 25°C as described in Materials and Methods. Inset: Scatchard plot for the [5F]Hir54–65(SO3−) interaction with prothrombin.
