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Competitive titration of the [5F]BJC–prothrombin complex with Hir54–65(SO3−). Titration with Hir54–65(SO3−) was performed in TBS containing 100 nM [5F]BJC and 250 nM prothrombin. Experiments were performed at 25°C, and polarization was measured as described in Materials and Methods. The solid line represents the nonlinear least-squares fit by the model for competitive binding of [5F]BJC and the unlabeled hirudin peptide to prothrombin, with the fitted parameters given in the text.
