Figure 2.

(A) Schematic drawing of the yeast two-hybrid system used to monitor the reconstitution of the N- and C-terminal fragments. The C-terminal fragment is fused to the LexA DNA binding domain and the N-terminal fragment is fused to the B42 activation domain. (B) Yeast two-hybrid β-galactosidase assay for all combinations of the N-and C-terminal fragments of FNfn10 produced in this work. The identity of a C-terminal fragment is indicated in each panel, and those of N-terminal fragments at the bottom of the figure. pTarget and pBait are two control proteins (Origene), which interact with each other.