Figure 5.

(A). Yeast two-hybrid β-galactosidase assays for the interactions of the wild-type ABC fragment and different DEFG fragments (bar graph). Yeast two-hybrid survival assays of the cells containing the indicated fragment pair in minimal media lacking leucine (middle column). The right column shows ΔΔG of the uncut protein, defined as ΔG_Mutant − ΔG_WT. (B) β-Galactosidase assays for the interactions of mutant ABC fragments isolated from yeast two-hybrid screening with the DEFG(I59A) fragment.