Table 1.
Sequences of selected clones from yeast two hybrid screening of FNfn10 ABC fragment library against C-terminal fragment DEFG(I59A) and their effects on the stability of the wild-type protein and I59A variant
| Position | ||||||
| Clone | 23 | 28 | 36 | 37 | Δ ΔG (WT) (kcal mol−1) | Δ ΔG (I59A) (kcal mol−1) |
| WT | D | T | Y | G | 0 | 0 |
| 1 | D | T | F | R | −0.1 ± 0.2 | 0.7 ± 0.1 |
| 2 | D | T | C | E | −0.5 ± 0.2 | 0.2 ± 0.1 |
| 3 | V | N | C | W | 1.5 ± 0.2 | 2.1 ± 0.5 |
| 4 | D | T | Y | E | −0.3 ± 0.2 | 0.1 ± 0.1 |
Mutated residues are shown in bold. Δ ΔG(WT) is defined as ΔGmutant − ΔGWT and Δ ΔG(I59A) is defined as ΔGmutant − ΔGI59A. The errors associated with ΔG values are the standard deviations determined from independent experiments.