Table 1.

Dynamic light scattering analysis of BFP-labeled EGFR-ICD proteins

Sample	Rh (nm)	Mr (kDa)	% Mass intensity
EGFR-ICD	4.34 ± 0.14	104	93
EGFR-ICD~Pa	4.35 ± 0.17	105	91
EGFR-Δ976	3.70 ± 0.70	72	91
EGFR-Δ976~Pa	3.64 ± 0.53	69	92
EGFR-Δ1022	3.56 ± 0.75	66	89
EGFR-Δ1022~Pa	3.62 ± 0.69	68	88

Protein samples were analyzed by use of a DynaPro Molecular Sizing Instrument and Dynamics software. The actual translation diffusion coefficient measured for each sample was used to estimate the hydrodynamic radius (R_h) based on the Stokes-Einstein equation, and the molecular mass (M_r) was estimated by using the supplied standard curve of M_r versus R_h data. The percent mass intensity for each sample was calculated after subtraction of solvent scattering peaks. R_h values are given as the average ± SE of four independent determinations.

^a Denotes the BFP-labeled proteins under phosphorylating conditions.