Figure 1.

Crude ATR/FTIR absorption spectra of hen lysozyme. The transmission spectra of 0.01 M sodium phosphate buffer pH = 7.0 and of lysozyme solutions at different concentrations in the same buffer were recorded in single beam mode. The corresponding absorption spectra were constructed using the buffer spectrum as the 100% reference. For each protein concentration, the absorbance (in arbitrary units) is shown as a function of the light frequency. The lysozyme concentrations were 1, 2, 4, 6, and 8 mg/mL, respectively, for the spectra in the order of increasing peak height.