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ATR/FTIR absorption spectrum of crystal-adsorbed lysozyme. Lysozyme (20 μL) at 8 mg/mL was incubated for 10 min in the ATR well, carefully removed by aspiration under vacuum, and replaced with 20 μL of buffer. The transmission spectrum was recorded in single beam mode with 100 accumulations. The absorption spectrum shown was then constructed using the transmission spectrum of pure buffer as the 100% reference.
