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Catalytic efficiency (Vmax/Km ratios calculated from the data reported in Table 2) of wild type and mutants of VAC. The values have been determined on CephC (A) and gl-7ACA (B) as substrates, and the ratio of the specificity constants (C) on the two substrates [(Vmax/Km)CephC/(Vmax/Km)gl-7ACA] between the mutants and the wild-type VAC.
