Table 3.
Site-saturation mutagenesis of VAC variants
| Starting material | Amino acid position | No. of selected mutants | Selected mutants |
| Wild type | 215 | 250 | E, F, L, V |
| 261 | 230 | — | |
| 263 | 200 | — | |
| 271 | 242 | V, F | |
| 294 | 200 | ||
| 296 | 220 | N, S, T, F | |
| 307 | 220 | — | |
| 308 | 200 | — | |
| 309 | 220 | Y, S | |
| 416 | 290 | — | |
| 417 | 180 | Y | |
| H296S | 215 | 210 | Y |
| 271 | 200 | — | |
| 294 | 200 | — | |
| 297 | 200 | — | |
| 417 | 200 | M | |
| A215Y-H296S | 416 | 220 | — |
| D416Y-H417Y | 271 | 200 | — |
| H417Y | 263 | 215 | — |
The positions subjected to saturation mutagenesis were identified by modeling of CephC to the active site of the VAC model (see Fig. 2 ▶).