Figure 2.

¹H/¹³C-HSQC spectra of MurF in the presence and the absence of compound 1 and ATP. (A) Spectra of ¹³CH₃ (methyl)-labeled MurF are contoured in the presence (red) or the absence (black) of compound 1. (B) MurF spectra are contoured in the presence (red) or the absence (black) of ATP. The green boxes connected in A and B highlight peaks that are significantly perturbed upon addition of ATP but not compound 1, while the blue boxes connected in A and B highlight peaks that are significantly perturbed upon addition of compound 1 but not ATP, indicating two different binding sites.