Figure 3.

pH dependence of Pce kinetic constants for the hydrolysis of NPPC. Measurements were carried out at 25°C in the buffers indicated in the experimental section supplemented with 3 μMZn²⁺ (I = 0.05). The continuous curves are the fittings of equations derived for the two deprotonation-state model (Fig. 4 ▶) to the experimental data with the parameters shown in Table 4. (A) Logarithmic plot of k_cat. (B) Logarithmic plot of k_cat/K_m. (C) Logarithmic plot of K_m.